Molecular and Biochemical Characterization of Sachharomyces Cerevisiae Actin Related Proctein 8 (ARP8)

PI:Prof. Saravanan Matheshwaran

The eukaryotic genome is packaged into nucleosomes, in which the DNA is wrapped around histone octamers and forms higher order chromatin. Alteration of chromatin structure is essential for DNA transaction processes like replication, transcription, recombination and repair. It has been shown that Actin Related Proteins (ARPs) are part of INO80 chromatin remodeling complex, the only known complex have role in DNA damage recognition and repair. Several studies have demonstrated that access to the DNA damage buried in nucleosome core is essential to repair the DNA damage.  Recent studies have shown that Arp8 can binds to core histone and hypothesized that it may have role in nucleosome assembly and disassembly during and after the repair. However it is not clear, how these complexes have such role and what is the molecular function for Arp8. The project will help us to understand the molecular and biochemical function of Arp8 on its own or with other interacting proteins.