CHM632 - Enzyme- reactions mechanism and kinetics
Prerequisites: CHM 481
3-0-0 (9)
Course Contents
Enzyme kinetics of single and multiple substrate systems including Enzyme assays and inhibition
(15 lectures)
Cooperativity and multienzyme systems (4 lectures)
Enzyme structure and identification of active site residues labelin, chemical modification and mutagenesis
(6 lectures)
Enzyme Mechanisms – Methods of study and mechanisms of some enzymes like
Serine proteases, polymerases, ribonucleases, lysozyme and ribonucleotide reductases (radical enzyme)
(15 lectures)
Mechanism based enzyme inhibition and drugs –5-fluorouracil for thymidylate synthase
(2 lectures)
Topics
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Books and References
Allan Ferhst, Structure and Mechanism in Protein Science: A Guide to Enzyme Catalysis and Protein Folding.
N. C. Price and E. Stevens, Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins.
I. H. Segel, Biochemical calculations, How to Solve Mathematical Problems in General Biochemistry, 2nd Edition.