BSE621A - Modern Instrumental Methods in Biological Sciences

Applications of spectroscopic and other techniques to the study of bio molecules: UVVis spectroscopy, Circular dichroism, Fluorescence, NMR, Mass, IR and Raman spectroscopy, XRay diffraction. Cellular Imaging Techniques: Microscopy: Phase contrast Nomarsky, Fluorescence, Atomic Force and con focal. Biophysical techniques to purify and study proteins. Dialysis, salting out and precipitation by organic solvents, Ion exchange, gel filtration, reversed phase, affinity chromatography, ultra centrifugation, gel electrophoresis. Analysis of Proteins: Electro-phonetic separation of proteins (single dimension native and denaturing gels, 2D and digital electro-phonetic analysis), detection (staining, blotting and immuno detection) and purification (various chromatograpy, HPLC, immuno precipitation)of proteins, and specialized applications (in vitro synthesis of protein, labeling, micro sequence analysis, and Mas spectrometry)